Institut für Systembiotechnologie

Synthetic bioengineering of Corynebacterium glutamicum as industrial cell factory

The soil bacterium Corynebacterium glutamicum is used for the industrial manufacture of products worth several billion euros annually. With a global market of about 1.5 million tons per year, L-lysine is one of its most important industrial products. L-lysine is mainly used for its beneficial properties as additive in animal and human nutrition. In addition, targeted genetic modification of C. glutamicum enables the synthesis of other added-value products such as the protective substance ectoine and the polymer block diaminopentane. This wide range of products has substantially boosted the economic importance of C. glutamicum. Here, we work on tailor-made strains of C. glutamicum for the industrial production of chemicals: with new methods of systems biotechnology and synthetic biotechnology, we generate next generation producers with superior properties.

 

  • Systems biology of C. glutamicum by 13C fluxomics, metabolomics, transcriptomics
  • In-silico pathway design
  • Rational design of production strains to increase production rates and the synthesis of new value-added products
  • Sustainable production of bio-based products from renewable resources

Publications

Gießelmann, G, Dietrich, D, Jungmann, L, Kohlstedt, M, Jeon, EJ, Yim, SS, Sommer, F, Zimmer, D, Mühlhaus, T, Schroda, M, Jeong, KJ, Becker, J, Wittmann, C (2019) Metabolic engineering of Corynebacterium glutamicum for high-level ectoine production – design, combinatorial assembly and implementation of a transcriptionally balanced heterologous ectoine pathway. Biotechnol. J. 14:e1800417. Link.

Becker, J, Rohles, CM, Wittmann, C (2018) Metabolically engineered Corynebacterium glutamicum for bio-based production of chemicals, fuels, materials, and healthcare products. Metab. Eng. 50:122-141. Link.

Hoffmann, SL, Jungmann, L, Schiefelbein, S, Peyriga, L, Cahoreau, E, Portais, JC, Becker, J, Wittmann, C (2018) Lysine production from the sugar alcohol mannitol: Design of the cell factory Corynebacterium glutamicum SEA-3 through integrated analysis and engineering of metabolic pathway fluxes. Metab. Eng. 47:475-487. Link.

Becker J, Zelder O, Häfner S, Schröder H, Wittmann C (2011) From zero to hero - design-based systems metabolic engineering of Corynebacterium glutamicum for L-lysine production. Metab. Eng. 13:159-168. Link.